Journal: Respiratory Research

Article Title: Different expression levels of interleukin-35 in asthma phenotypes

doi: 10.1186/s12931-020-01356-6

Figure Lengend Snippet: Correlations between sputum IL-35 and clinical characteristics

Article Snippet: Quantitative detection of sputum IL-35 was performed using the commercial human interleukin-35 (IL-35) ELISA kit (Catalog Number CSB-E13126h, CUSABIO, China) in accordance with the manufacturer’s protocol.

Techniques:

Journal: PeerJ

Article Title: Malignant behaviors and immune response in melanoma: Epstein-Barr virus induced gene 3 as a therapeutic target based on an in-vitro exploration

doi: 10.7717/peerj.18730

Figure Lengend Snippet: (A–C) Quantification on the phosphorylation of SMAD3 and STAT6 in EBI3 -silenced melanoma cells MV3 via western blotting. (D) Measurement on the immune response-related cytokines IL-35, IL-4 and IL-13 in EBI3 -silenced melanoma cells MV3 via ELISA. * p < 0.05; ** p < 0.01.

Article Snippet: In this study, melanoma cells were cultured in the six-well culture plates at the density of 8 × 10 5 /well for 24 h. Hereafter, the cell culture supernatant was harvested for the calculation on the levels of relevant cytokines including IL-35 (NBP3-06774), IL-4 (NBP1-91171) and IL-13 (NBP1-91176) as per the manuals of the producer (Novus Biologicals, Centennial, CO, USA).

Techniques: Phospho-proteomics, Western Blot, Enzyme-linked Immunosorbent Assay